The construction of Application-oriented Colleges and universities is a new mission entrusted to higher education by economic and social development. At present, there are a series of problems in the construction of application-oriented universities, such as inadequate understanding of the connotation of application-oriented universities, unclear development orientation, and ineffective evaluation methods. It can effectively strengthen the construction of Application-oriented Colleges and universities by inheriting and carrying forward the excellent tradition of running schools, defining the development goals and positioning, constructing the discipline and specialty system, and improving the connotation construction level of colleges and universities.