The paper aims to investigate the status quo of professional identity of nursing graduates and analyze the related factors affecting their professional identity. During March 2019-September 2019, the professional identity questionnaire of nursing staff was used to investigate the professional identity of 320 nursing master graduates in Hubei Province, and the general information was investigated by general situation questionnaire. The total score of professional identity of 320 nursing master graduates in Hubei province was (36.11 ± 5.49). Logistic regression analysis showed that occupational risk, social status and professional title were the main factors affecting their professional identity (P < 0.05). The professional identity of nursing master graduates in Hubei Province is low, mainly affected by occupational risk, social status and professional title.